Faculty Research 1980 - 1989
Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.
Document Type
Article
Publication Date
1986
Keywords
Animal, Base-Sequence, Cell-Adhesion, Cloning-Molecular, Comparative-Study, DNA: ge, Fluorescent-Antibody-Technique, Genes-Structural, Glycoproteins: ge, im, Immunosorbent-Techniques, Mice, Repetitive-Sequences-Nucleic-Acid, RNA-Messenger: ge, Sequence-Homology-Nucleic-Acid, SUPPORT-NON-U-S-GOVT
First Page
1364
Last Page
1368
JAX Source
Proc Natl Acad Sci U S A 1986 Mar;83(5):1364-8
Abstract
A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.
Recommended Citation
Schuh R,
Vestweber D,
Riede I,
Ringwald M,
Rosenberg UB,
Jackle H,
Kemler R.
Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence. Proc Natl Acad Sci U S A 1986 Mar;83(5):1364-8