Faculty Research 1980 - 1989

Title

Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.

Document Type

Article

Publication Date

1986

Keywords

Animal, Base-Sequence, Cell-Adhesion, Cloning-Molecular, Comparative-Study, DNA: ge, Fluorescent-Antibody-Technique, Genes-Structural, Glycoproteins: ge, im, Immunosorbent-Techniques, Mice, Repetitive-Sequences-Nucleic-Acid, RNA-Messenger: ge, Sequence-Homology-Nucleic-Acid, SUPPORT-NON-U-S-GOVT

JAX Source

Proc Natl Acad Sci U S A 1986 Mar;83(5):1364-8

Abstract

A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.

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