Faculty Research 1990 - 1999

A specific, nonproliferative role for E2F-5 in choroid plexus function revealed by gene targeting.

Document Type

Article

Publication Date

1998

Keywords

Alleles, Animal, Cell-Division, Choroid-Plexus, Gene-Deletion, Gene-Targeting, Human, Hydrocephalus, Mice, Mice-Knockout, Transcription-Factors

First Page

1092

Last Page

1098

JAX Source

Genes Dev 1998 Apr 15;12(8):1092-8

Abstract

Homozygous E2F-5 knockout embryos and mice have been generated. Although embryonic development appeared normal, newborn mice developed nonobstructive hydrocephalus, suggesting excessive cerebrospinal fluid (CSF) production. Although the CSF-producing choroid plexus displayed normal cellular organization, it contained abundant electron-lucent epithelial cells, consistent with excessive CSF secretory activity. Moreover, E2F-5 CNS expression in normal animals was largely confined to the choroid plexus. Cell cycle kinetics were not perturbed in homozygous knockout embryo fibroblasts. Thus, E2F-5 is not essential for cell proliferation. Rather, it affects the secretory behavior of a differentiated neural tissue.

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