Faculty Research 1990 - 1999
Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4.
Document Type
Article
Publication Date
1999
Keywords
Antibodies, Antigens-CD34: an, im, Antigens-Differentiation: an, Chemokines-CXC: ph, Chemotaxis, Colony-Forming-Units-Assay, Fetal-Blood, Hematopoietic-Stem-Cell-Mobilization, Hematopoietic-Stem-Cell-Transplantation, Hematopoietic-Stem-Cells: ph, Human, Interleukin-6, Mice, Mice-Inbred-NOD, Mice-Knockout, Mice-SCID, NAD+-Nucleosidase: an, Receptors-CXCR4: bi, im, ph, Stem-Cell-Factor, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Tetradecanoylphorbol-Acetate, Up-Regulation-(Physiology)
First Page
845
Last Page
848
JAX Source
Science 1999 Feb 5;283(5403):845-8
Grant
A130389
Abstract
Stem cell homing and repopulation are not well understood. The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 were found to be critical for murine bone marrow engraftment by human severe combined immunodeficient (SCID) repopulating stem cells. Treatment of human cells with antibodies to CXCR4 prevented engraftment. In vitro CXCR4-dependent migration to SDF-1 of CD34+CD38-/low cells correlated with in vivo engraftment and stem cell function. Stem cell factor and interleukin-6 induced CXCR4 expression on CD34+ cells, which potentiated migration to SDF-1 and engraftment in primary and secondary transplanted mice. Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation.
Recommended Citation
Peled A,
Petit I,
Kollet O,
Magid M,
Ponomaryov T,
Byk T,
Nagler A,
Ben HH,
Many A,
Shultz LD,
Lider O,
Alon R,
Zipori D,
Lapidot T.
Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 1999 Feb 5;283(5403):845-8