Faculty Research 1990 - 1999
Fine mapping of the friend retrovirus resistance gene, Rfv3, on mouse chromosome 15.
Document Type
Article
Publication Date
1999
Keywords
Chromosome-Mapping, DNA-Binding-Proteins, Female, Friend-Virus, Immunity-Natural, Male, Mice, Mice-Inbred-C57BL, Retroviridae-Infections, SUPPORT-U-S-GOVT-P-H-S, Tumor-Virus-Infections
First Page
7848
Last Page
7852
JAX Source
J Virol 1999 Sep; 73(9):7848-52.
Abstract
Rfv3 is a host resistance gene that operates through an unknown mechanism to control the development of the virus-neutralizing antibody response required for recovery from infection with Friend retrovirus. The Rfv3 gene was previously mapped to an approximately 20-centimorgan (cM) region of chromosome 15. More refined mapping was not possible, due to a lack of microsatellite markers and leakiness in the Rfv3 phenotype, which prevented definitive phenotyping of individual recombinant mice. In the present study, we overcame these difficulties by taking advantage of seven new microsatellite markers in the Rfv3 region and by using progeny tests to accurately determine the Rfv3 phenotype of recombinant mice. Detailed linkage analysis of relevant crossovers narrowed the location of Rfv3 to a 0.83-cM region. Mapping of closely linked genes in an interspecific backcross panel allowed us to exclude two previous candidate genes, Ly6 and Wnt7b. These studies also showed for the first time that the Hsf1 gene maps to the Rfv3-linked cluster of genes including Il2rb, Il3rb, and Pdgfb. This localization of Rfv3 to a region of less than 1 cM now makes it feasible to attempt the cloning of Rfv3 by physical methods.
Recommended Citation
Super HJ,
Hasenkrug KJ,
Simmons S,
Brooks DM,
Konzek R,
Sarge KD,
Morimoto RI,
Jenkins NA,
Gilbert DJ,
Copeland NG,
Frankel W,
Chesebro B.
Fine mapping of the friend retrovirus resistance gene, Rfv3, on mouse chromosome 15. J Virol 1999 Sep; 73(9):7848-52.