Faculty Research 1990 - 1999
Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice.
Document Type
Article
Publication Date
1999
Keywords
Asthma, Bronchial-Hyperreactivity, Bronchial-Provocation-Tests, Bronchoconstrictor-Agents, Chromosome-Mapping, Chromosomes, Genotype, Linkage-(Genetics), Male, Methacholine-Chloride, Mice, Mice-Inbred-A, Mice-Inbred-C3H, Quantitative-Trait, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
JAX Source
Am J Physiol 1999 Dec; 277(6 PT 1):L1118-23.
Grant
HL36110/HL/NHLBI
Abstract
Quantitative trait locus (QTL) mapping was used to identify chromosomal regions contributing to airway hyperresponsiveness in mice. Airway responsiveness to methacholine was measured in A/J and C3H/HeJ parental strains as well as in progeny derived from crosses between these strains. QTL mapping of backcross [(A/J x C3H/HeJ) x C3H/HeJ] progeny (n = 137-227 informative mice for markers tested) revealed two significant linkages to loci on chromosomes 6 and 7. The QTL on chromosome 6 confirms the previous report by others of a linkage in this region in the same genetic backgrounds; the second QTL, on chromosome 7, represents a novel locus. In addition, we obtained suggestive evidence for linkage (logarithm of odds ratio = 1.7) on chromosome 17, which lies in the same region previously identified in a cross between A/J and C57BL/6J mice. Airway responsiveness in a cross between A/J and C3H/HeJ mice is under the control of at least two major genetic loci, with evidence for a third locus that has been previously implicated in an A/J and C57BL/6J cross; this indicates that multiple genetic factors control the expression of this phenotype.
Recommended Citation
De SG,
Singer JB,
Jiao A,
Yandava CN,
Lee YH,
Haynes TC,
Lander ES,
Beier DR,
Drazen JM.
Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice. Am J Physiol 1999 Dec; 277(6 PT 1):L1118-23.