Faculty Research 1990 - 1999

Mapping genes encoding drug-metabolizing enzymes in recombinant inbred mice.

Document Type

Article

Publication Date

1991

Keywords

Chromosome-Mapping, Cytochrome-P-450: ge, DNA-Probes, Genes-Reiterated, Glucuronosyltransferase: ge, Glutathione-Transferases: ge, Human, Mice, Mice-Inbred-Strains: ge, Rats, Restriction-Fragment-Length-Polymorphisms, SUPPORT-U-S-GOVT-P-H-S

First Page

309

Last Page

316

JAX Source

Genomics 1991 Oct;11(2):309-16

Grant

GM18684, CA33093

Abstract

Probes for cytochrome P450IVA (P450IVA), alpha- and pi-class glutathione S-transferases (GST), and phenol-metabolizing UDP-glucuronyltransferase (UDPGT-K39) detected restriction fragment length variants (RFLVs) between C57BL/6J and DBA/2J mice. These variants were used to map the P450IVA genes (Cyp4 alpha) to chromosome 4, close to Mtv-13 and Pmv-19, midway between brown (b) and Gpd-1; GST alpha genes were mapped to chromosome 9, with a cross-hybridizing sequence mapping to another chromosome; the GST pi genes were mapped to the distal end of chromosome 1 near Pmv-21; one UDPGT-K39 variant to chromosome 1, between Acrg and Emv-17, and another showed linkage to Odc-10 on an unidentified chromosome. No RFLVs were detected with probes for P450IID, P450 reductase, androsterone-metabolizing UDPGT, GST mu, or microsomal GST.

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