Faculty Research 1990 - 1999
Mouse platelet-derived growth factor receptor alpha gene is deleted in W19H and patch mutations on chromosome 5.
Document Type
Article
Publication Date
1991
Keywords
Blotting-Southern, Chromosome-Deletion, Chromosome-Mapping, Cricetulus, DNA: ge, Female, Hamsters, Hybrid-Cells: cy, Linkage-(Genetics), Male, Mice, Mice-Inbred-C3H, Mice-Mutant-Strains: ge, Muridae, Mutation, Nucleic-Acid-Hybridization, Platelet-Derived-Growth-Factor: ph, Receptors-Cell-Surface: ge, Recombination-Genetic, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
4811
Last Page
4815
JAX Source
Proc Natl Acad Sci U S A 1991 Jun 1;88(11):4811-5
Grant
PO1AG06872/AG/NIA, HG00101/HG/NCHGR, DK27726/DK/NIDDK
Abstract
The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor alpha gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/ + heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.
Recommended Citation
Smith EA,
Seldin MF,
Martinez L,
Watson ML,
Choudhury GG,
Lalley PA,
Pierce J,
Aaronson S,
Barker JE,
Naylor SL,
Sakaguchi AY.
Mouse platelet-derived growth factor receptor alpha gene is deleted in W19H and patch mutations on chromosome 5. Proc Natl Acad Sci U S A 1991 Jun 1;88(11):4811-5