Faculty Research 1990 - 1999
Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins.
Document Type
Article
Publication Date
1992
Keywords
Apolipoproteins, Immunodiffusion: mt, Mice, Sensitivity-and-Specificity, Silver, Stains-and-Staining, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
1073
Last Page
1078
JAX Source
J Lipid Res 1992 Jul;33(7):1073-8
Grant
HL32087/HL/NHLBI
Abstract
Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. These advantages make the modified RID assay an attractive alternative to other forms of immunoassay.
Recommended Citation
Ishida BY,
Paigen B.
Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins. J Lipid Res 1992 Jul;33(7):1073-8