Faculty Research 1990 - 1999

The mouse Enhancer trap locus 1 (Etl-1): a novel mammalian gene related to Drosophila and yeast transcriptional regulator genes.

Document Type

Article

Publication Date

1992

Keywords

Amino-Acid-Sequence, Animal, Base-Sequence, Comparative-Study, Drosophila-melanogaster: ge, DNA: ge, Enhancer-Elements-(Genetics), Fetal-Development: ge, Genes-Structural, Genetic-Techniques, Mice: em, ge, Molecular-Sequence-Data, Open-Reading-Frames, Organ-Specificity, Proteins: bi, ge, Recombinant-Fusion-Proteins: bi, ge, RNA-Messenger: an, Saccharomyces-cerevisiae: ge, Sequence-Alignment, Sequence-Homology-Amino-Acid, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Transcription-Factors: bi, ge

First Page

111

Last Page

123

JAX Source

Mech Dev 1992 Nov;39(1-2):111-23

Grant

5R01HD2533403/HD/NICHD

Abstract

A novel mouse gene, Enhancer trap locus 1 (Etl-1), was identified in close proximity to a lacZ enhancer trap integration in the mouse genome showing a specific beta-galactosidase staining pattern during development. In situ analysis revealed a widespread but not ubiquitous expression of Etl-1 throughout development with particularly high levels in the central nervous system and epithelial cells. The amino acid sequence of the Etl-1 protein deduced from the cDNA shows strong similarity, over a stretch of 500 amino acids, to the Drosophila brahma protein involved in the regulation of homeotic genes and to the yeast transcriptional activator protein SNF2/SWI2 as well as to the RAD54 protein and the recently described helicase-related yeast proteins STH1 and MOT1. Etl-1 is the first mammalian member of this group of proteins that are implicated in gene regulation and/or influencing chromatin structure. The homology to the regulatory proteins SNF2/SWI2 and brahma and the expression pattern during embryogenesis suggest that Etl-1 protein might be involved in gene regulating pathways during mouse development.

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