Faculty Research 1990 - 1999
Mutations at the murine motheaten locus are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene.
Document Type
Article
Publication Date
1993
Keywords
Base-Sequence, Chromosome-Mapping, Comparative-Study, Hematopoiesis: ge, Hematopoietic-Stem-Cells, Macrophages, Mice, Mice-Inbred-Strains, Molecular-Sequence-Data, Mutagenesis-Site-Directed, Mutation, Protein-Tyrosine-Phosphatase: ge, RNA-Splicing, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
1445
Last Page
1454
JAX Source
Cell 1993 Jul 2;73(7):1445-54
Grant
CA20408/CA/NCI, AI30389/AI/NIAID, AI2773/AI/NIAID, +
Abstract
Mice homozygous for the recessive allelic mutation motheaten (me) or viable motheaten (mev) on chromosome 6 develop severe defects in hematopoiesis. In this paper we present the findings that the me and mev mutations are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene. High resolution mapping localized me to an area tightly linked to Hcph on chromosome 6. Abnormalities of the Hcph protein product were demonstrated by Western blot analysis and by activity assays in both me/me and mev/mev mice. Molecular analysis of the Hcph cDNA identified abnormal transcripts in both mutants. DNA sequence analyses of cDNA and genomic clones revealed that both the me and mev mutations are point mutations that result in aberrant splicing of the Hcph transcript. These findings provide the first available animal models for a specific protein-tyrosine phosphatase deficiency, thus facilitating determination of the precise role of this signaling molecule in hematopoiesis.
Recommended Citation
Shultz LD,
Schweitzer PA,
Rajan TV,
Yi T,
Ihle JN,
Matthews RJ,
Thomas ML,
Beier DR.
Mutations at the murine motheaten locus are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene. Cell 1993 Jul 2;73(7):1445-54