Faculty Research 1990 - 1999
Cloning, analysis, and chromosomal localization of Notch-1, a mouse homolog of Drosophila Notch.
Document Type
Article
Publication Date
1993
First Page
259
Last Page
264
JAX Source
Genomics 1993 Feb;15(2):259-64
Grant
NO1-CO-74101/CO/NCI
Abstract
The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.
Recommended Citation
del Am,
Gendron MM,
Swiatek PJ,
Jenkins NA,
Copeland NG,
Gridley T.
Cloning, analysis, and chromosomal localization of Notch-1, a mouse homolog of Drosophila Notch. Genomics 1993 Feb;15(2):259-64