Faculty Research 1990 - 1999

Genetic mapping of 18S ribosomal RNA-related loci to mouse chromosomes 5, 6, 9, 12, 17, 18, 19, and X.

Document Type

Article

Publication Date

1996

Keywords

Chromosome-Mapping, DNA-Ribosomal, Female, Male, Mice, Mice-Inbred-C57BL, RNA-Ribosomal-18S, SUPPORT-U-S-GOVT-P-H-S, X-Chromosome

First Page

886

Last Page

889

JAX Source

Mamm Genome 1996 Dec;7(12):886-9

Abstract

The organization of ribosomal RNA genes (rDNA) in the genome of the mouse varies significantly from one strain to another, but has been shown to follow the pattern of clusters of tandem repeats located at chromosome ends, often associated with cytological nucleolus organizer regions. The number of copies of the repeat unit at each locus also varies. A probe for the 18S ribosomal RNA sequence on Southern blots reveals both high copy number bands and fainter bands indicative of low repeat number. We have mapped a number of newly identified low-copy-number rDNA loci in C57BL/6J, in addition to placing some of the NOR-associated rDNA repeats on the Jackson interspecific backcross (BSS) map. We suggest that additional low-copy-number loci may remain to be mapped, and that the evolution of rDNA loci in the genome may include the proliferation of single copies by retroinsertion or other mechanisms.

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