Faculty Research 1990 - 1999
Expression and cell membrane localization of catenins during mouse preimplantation development.
Document Type
Article
Publication Date
1996
Keywords
Blastocyst: me, Cadherins: bi, Cell-Membrane: me, Cytoskeletal-Proteins: bi, Fetal-Development, Mice, SUPPORT-U-S-GOVT-P-H-S
First Page
391
Last Page
402
JAX Source
Dev Dyn 1996 Aug;206(4):391-402
Abstract
We have studied transcription, expression, and membrane localization of components of the E-cadherin-catenin complex stage by stage during mouse preimplantation development. Maternal E-cadherin and alpha- and beta-catenin are stored as mRNA and/or protein in unfertilized eggs and are already assembled into a protein complex at this stage. After fertilization, it is likely that they mediate adhesion of early-stage blastomeres. Biosynthesis of plakoglobin is delayed relative to the other components. The temporal mRNA and protein expression patterns of the components of the cadherin-catenin complex correlate with the presence or absence of potential cytoplasmic polyadenylation elements (CPEs) in the 3'-UTRs of the respective cDNAs. Our results suggest that the components of the E-cadherin-catenin complex derived from both maternal and zygotic gene activity are increasingly accumulated and stored in a nonfunctional form during early cleavage stages and are ready to be used for compaction and the formation of the trophectodermal cell layer.
Recommended Citation
Ohsugi M,
Hwang SY,
Butz S,
Knowles BB,
Solter D,
Kemler R.
Expression and cell membrane localization of catenins during mouse preimplantation development. Dev Dyn 1996 Aug;206(4):391-402