Faculty Research 1990 - 1999
Glycosylphosphatidylinositol-anchored H-2Db molecules are defective in antigen processing and presentation to cytotoxic T lymphocytes.
Document Type
Article
Publication Date
1996
Keywords
Antigen-Presentation, Cells-Cultured, Cytotoxicity-Immunologic, Glycosylphosphatidylinositols: an, ph, H-2-Antigens: an, ph, Male, Mice, Mice-Inbred-CBA, Mice-Inbred-C3H, Mice-Inbred-C57BL, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes-Cytotoxic: im, Transfection
First Page
2215
Last Page
2224
JAX Source
Eur J Immunol 1996 Sep;26(9):2215-24
Grant
GM46467/GM/NIGMS, DK38452/DK/NIDDK, AI24562/AI/NIAID
Abstract
Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These same transfectants can be lysed by allospecific CTL, or by antigen-specific Db-restricted CTL when pulsed with appropriate exogenous synthetic peptide, demonstrating that they can bind and present peptide for CTL-mediated lympholysis. The defect apparently results from differences between GPI-Db and TM-Db assembly and transport, or from differences in membrane topology that affect CD8+ CTL recognition of major histocompatibility complex/peptide complex.
Recommended Citation
Cariappa A,
Flyer DC,
Rollins CT,
Roopenian DC,
Flavell RA,
Brown D,
Waneck GL.
Glycosylphosphatidylinositol-anchored H-2Db molecules are defective in antigen processing and presentation to cytotoxic T lymphocytes. Eur J Immunol 1996 Sep;26(9):2215-24