Faculty Research 1990 - 1999
Genetic mapping of 20 novel expressed sequence tags from midgestation mouse embryos suggests chromosomal clustering.
Document Type
Article
Publication Date
1997
Keywords
Base-Sequence, Chromosome-Mapping, DNA-Complementary, Embryo, Female, Gene-Expression, Male, Mice, Mice-Inbred-C57BL, Molecular-Sequence-Data, Multigene-Family, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
170
Last Page
174
JAX Source
Genomics 1997 Feb 15;40(1):170-4
Grant
AI39651/AI/NIAID, AI39824/AI/NIAID
Abstract
Current knowledge of genes that regulate pattern formation and differentiation processes during mammalian embryonic development is limited. In an effort to isolate developmentally relevant genes, 20 novel, end-sequenced cDNAs selected from a Day 10.5 postcoitum mouse embryo library were genetically mapped in intersubspecific backcross mice. Eleven of 20 cDNA clones mapped to three mouse autosomes (Chr 5, 11, and 14), a result that was unlikely (P < 0.03) if the distribution of genes expressed in embryos is random within the mouse genome. Several clones were candidates for mouse developmental mutations by virtue of genetic colocalization and concordance of embryonic expression patterns with the distribution of defects in mutant mice: Estm11 was a candidate for the mouse mutation wabbler-lethal (wl), since Estm11 mapped in the vicinity of wl on mouse Chr 14 and was expressed in those regions of embryonic brain that exhibit axonal degeneration in wl. End-sequence analysis, genetic mapping, and in situ hybridization appeared to be an effective combination of methods for identification and characterization of genes with potential regulatory functions during mammalian embryogenesis.
Recommended Citation
Tchernev VT,
Barbosa MD,
Detter JC,
Patel TD,
Achey K,
Wakeland EK,
Gueorguieva RV,
Yang MC,
Gossler A,
Kingsmore SF.
Genetic mapping of 20 novel expressed sequence tags from midgestation mouse embryos suggests chromosomal clustering. Genomics 1997 Feb 15;40(1):170-4