Faculty Research 1990 - 1999

Identification and genetic mapping of a new polycystic kidney disease on mouse chromosome 8.

Document Type

Article

Publication Date

1997

Keywords

Chromosome-Mapping, Kidney-Polycystic: ge, pa, Mice, Mice-Inbred-C3H, Mice-Inbred-C57BL, Phenotype, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

First Page

101

Last Page

107

JAX Source

Genomics 1997 Feb 15;40(1):101-7

Grant

P40RR01183/RR/NCRR, GM46697/GM/NIGMS, DK49634/DK/NIDDK

Abstract

We report here a new mouse mutation, kat, that causes pleiotropic effects including facial dysmorphism, dwarfing, male sterility, anemia, and progressive polycystic kidney disease. kat (kidney anemia and testis) and a second allele, kat2J, that occurred on C57BL/ 6J were mapped to mouse chromosome (Chr) 8 using intra- and intersubspecific intercrosses. A high-resolution map for kat2J on Chr 8 was constructed using the F2 progeny from a cross between C57BL/6J-kat2J/+ and an inbred strain of Mus musculus castaneus (CAST/Ei). The kat2J mutation was localized between D8Mit129 and D8Mit128 with the gene order centromere-D8Mit100-(1.2 +/- 0.26 cM)-D8Mit231-(0.17 +/- 0.09 cM)-D8Mit129-(0.28 +/- 0.12 cM)-D8Mit128-(0.98 +/- 0.23 cM)-D8Mit25/D8Mit8. This segment is homologous to human Chr 19p. The two mutations at this locus that have occurred at The Jackson Laboratory will be invaluable for positional cloning and subsequent functional analysis of the mutated gene.

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