Faculty Research 1990 - 1999
Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice.
Document Type
Article
Publication Date
1997
Keywords
Biological-Markers, Cell-Line, Crosses-Genetic, Embryo: cy, Female, Graft-Rejection: ge, Male, Mice, Mice-Inbred-C3H, Mice-Inbred-C57BL, Mice-Inbred-Strains: ge, Mice-Mutant-Strains, Mutagenesis, Polymorphism-(Genetics), Proteins: ge, Skin-Transplantation, Stem-Cells: cy, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Variation-(Genetics)
First Page
19
Last Page
27
JAX Source
Nat Genet 1997 May;16(1):19-27
Abstract
Targeted mutagenesis in mice, a powerful tool for the analysis of gene function and human disease, makes extensive use of 129 mouse substrains. Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability among substrains and embryonic stem cells derived from them. This clearer understanding of 129 substrain variability allows consideration of its negative impact on targeting technology, including: homologous recombination frequencies, preparation of inbred animals, and availability of appropriate controls. Based on these considerations we suggest a number of recommendations for future experimental design.
Recommended Citation
Simpson EM,
Linder CC,
Sargent EE,
Davisson MT,
Mobraaten LE,
Sharp JJ.
Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat Genet 1997 May;16(1):19-27