Faculty Research 1990 - 1999

Multiplicity of uses of monoclonal antibodies that define papillomavirus linear immunodominant epitopes.

Document Type

Article

Publication Date

1997

Keywords

Animal, Antibodies-Monoclonal: du, Epitope-Mapping, Human, Immunodominant-Epitopes: im, Molecular-Sequence-Data, Papillomavirus-Bovine: im, Papovaviridae-Infections: im, di, Tumor-Virus-Infections: im, di

First Page

115

Last Page

119

JAX Source

Immunol Res 1997 Feb;16(1):115-9

Abstract

During the last 10 yr, we have derived monoclonal antibodies from animals immunized with denatured bovine papillomaviruses type 1 major capsid (L1) protein, mapped their corresponding immunodominant epitopes to within a single amino acid (aa), and compared the reactivity of authentic L1 proteins to the predicted response by collinear analysis of the aa sequences of the same and other papillomaviruses (PVs). The data obtained from this approach has provided us with new insights into the sensitivity and specificity of the antibody response to viral proteins. We have included here some observations and conclusions that appear to be generic for the immune response, some of which might have applications for working with linear epitopes in other experimental systems.

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