Equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the EcPV-1 L1 major capsid protein.

Document Type

Article

Publication Date

2004

Keywords

Animals, Antibodies-Monoclonal, Baculoviridae, Capsid-Proteins, Cell-Line, DNA, Electrophoresis-Polyacrylamide-Gel, Enzyme-Linked-Immunosorbent-Assay, Epitopes, Genome-Viral, Horses, Immunoblotting, Insects, Mice-Inbred-BALB-C, Oncogene-Proteins-Viral, Open-Reading-Frames, Papillomavirus, Phylogeny, Protein-Conformation, Recombinant-Proteins, Recombination-Genetic, Sequence-Analysis-DNA, Virion

First Page

1108

Last Page

1115

JAX Source

Biochem Biophys Res Commun 2004 Nov; 324(3):1108-15.

Abstract

Equus caballus papillomavirus type 1 (EcPV-1) was isolated from a cutaneous papilloma, the most common neoplasm in horses. The complete EcPV-1 nucleotide sequence and genomic organization were determined. Phylogenetic analysis showed that EcPV-1 is a close-to-root papillomavirus, with only distant relationships to the fibropapillomaviruses and the benign cutaneous papillomaviruses. To produce EcPV-1 virus-like particles (VLPs), the EcPV-1 L1 major capsid protein was expressed in insect cells using a recombinant baculovirus vector. The self-assembled EcPV-1 VLPs were morphologically indistinguishable from wild type papillomavirus virions. Monoclonal antibodies were developed against intact and denatured EcPV-1 VLPs. When tested by ELISA, all monoclonal antibodies produced against intact (#18) and some against denatured EcPV-1 VLPs (#16) reacted with intact EcPV-1 VLPs only, demonstrating that the VLPs carry type-specific conformational as well as linear epitopes on their surface. Recombinant EcPV-1 VLPs offer the potential of a noninfectious vaccine to prevent and eradicate equine cutaneous papillomatosis.

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