Genetic and physical mapping of the cerebellar deficient folia (cdf) locus on mouse chromosome 6 [In Process Citation]
Document Type
Article
Publication Date
2000
First Page
135
Last Page
138
JAX Source
Genomics 2000 Oct; 69(1):135-8.
Abstract
Cerebellar deficient folia (cdf) is a recessive mouse mutation causing ataxia and cerebellar cytoarchitectural abnormalities, including hypoplasia, foliation defects, and Purkinje cell ectopia. To identify the cdf gene, we have generated a high-resolution genetic map of a 3.24 +/- 0.55 cM (95% CI) region encompassing the cdf gene using 1997 F2 mice generated from a (C3H/HeSnJ-cdf/cdf x CAST/Ei)F1 intercross. Linkage analysis showed that the cdf gene cosegregates with D6Mit208, D6Mit359, and D6Mit225. A contig of five YACs, nine BACs, and three P1s was constructed across the cdf nonrecombinant region. Based on genetic and physical maps, the cdf gene was localized to the 0.28 +/- 0.23 cM (95% CI) interval between D6Mit209 and D6Ack1. These results will greatly facilitate the map-based cloning of the cdf gene, which in turn should further knowledge of the molecular mechanisms of neuronal positioning and foliation during cerebellar development. Copyright 2000 Academic Press.
Recommended Citation
Park C,
Longo CM,
Ackerman SL.
Genetic and physical mapping of the cerebellar deficient folia (cdf) locus on mouse chromosome 6 [In Process Citation] Genomics 2000 Oct; 69(1):135-8.