Isolation and cloning of the raccoon (Procyon lotor) papillomavirus type 1 by using degenerate papillomavirus-specific primers.
Document Type
Article
Publication Date
2005
Keywords
Cloning-Molecular, DNA-Primers, DNA-Viral, Genome-Viral, Molecular-Sequence-Data, Papillomavirus, Papillomavirus-Infections, Phylogeny, Polymerase-Chain-Reaction, Raccoons, Sequence-Analysis-DNA, Viral-Proteins
First Page
2029
Last Page
2033
JAX Location
see Reprint collection, a pdf is available.
JAX Source
J Gen Virol 2005 Jul; 86(7):2029-33.
Abstract
Partial sequences of a novel papillomavirus were amplified from a cutaneous lesion biopsy of a raccoon (Procyon lotor), by using PCR with degenerate papillomavirus-specific primers. The Procyon lotor papillomavirus type 1 (PlPV-1) DNA was amplified with long template PCR in two overlapping fragments, together encompassing the entire genome, and the complete PlPV-1 genomic sequence was determined. The PlPV-1 genome consists of 8170 bp, and contains the typical papillomaviral open reading frames, encoding five early proteins and two late capsid proteins. Besides the classical non-coding region (NCR1) between the end of L1 and the start of E6, PlPV-1 contains an additional non-coding region (NCR2) of 1065 bp between the early and late protein region, which has previously also been described for the canine oral papillomavirus (COPV) and the Felis domesticus papillomavirus (FdPV-1). Phylogenetic analysis places PlPV-1 together with COPV and FdPV-1 in a monophyletic branch which encompasses the Lambda papillomavirus genus.
Recommended Citation
Rector A,
Van DK,
Bertelsen M,
Barker IK,
Olberg RA,
Lemey P,
Sundberg JP,
Van RM.
Isolation and cloning of the raccoon (Procyon lotor) papillomavirus type 1 by using degenerate papillomavirus-specific primers. J Gen Virol 2005 Jul; 86(7):2029-33.