Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice.
Document Type
Article
Publication Date
2006
Keywords
Bone-and-Bones, Female, Femur, Humans, Male, Mice-Knockout, Osteogenesis, Parathyroid-Hormone, Receptor-Fibroblast-Growth-Factor-Type-2, Recombinant-Proteins, Research-Support-N, I, H, -Extramural, Teriparatide, Tomography-X-Ray-Computed
First Page
989
Last Page
994
JAX Source
Biochem Biophys Res Commun 2006; 341(4):989-94.
Abstract
Since parathyroid hormone (PTH) increased FGF2 mRNA and protein expression in osteoblasts, and serum FGF-2 was increased in osteoporotic patients treated with PTH, we assessed whether the anabolic effect of PTH was impaired in Fgf2-/- mice. Eight-week-old Fgf2+/+ and Fgf2-/- male mice were treated with rhPTH 1-34 (80mug/kg) for 4 weeks. Micro-CT and histomorphometry demonstrated that PTH significantly increased parameters of bone formation in femurs from Fgf2+/+ mice but the changes were smaller and not significant in Fgf2-/- mice. IGF-1 was significantly reduced in serum from PTH-treated Fgf2-/- mice. DEXA analysis of femurs from Fgf2+/+, Fgf2+/-, and Fgf2-/- mice treated with rhPTH (160mug/kg) for 10 days showed that PTH significantly increased femoral BMD in Fgf2+/+ by 18%; by only 3% in Fgf2+/- mice and reduced by 3% in Fgf2-/- mice. We conclude that endogenous Fgf2 is important for maximum bone anabolic effect of PTH in mice.
Recommended Citation
Hurley MM,
Okada Y,
Xiao L,
Tanaka Y,
Ito M,
Okimoto N,
Nakamura T,
Rosen CJ,
Doetschman T,
Coffin JD.
Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice. Biochem Biophys Res Commun 2006; 341(4):989-94.