4Pi microscopy of quantum dot-labeled cellular structures.
Document Type
Article
Publication Date
2006
Keywords
Cell-Line, Microscopy-Confocal, Microscopy-Fluorescence-Multiphoton, Microtubules, Mitochondria, Quantum-Dots, Reproducibility-of-Results
First Page
517
Last Page
523
JAX Source
J Struct Biol 2006 Dec; 156(3):517-23.
Abstract
The most prominent restrictions of fluorescence microscopy are the limited resolution and the finite signal. Established conventional, confocal, and multiphoton microscopes resolve at best approximately 200nm in the focal plane and only 500nm in depth. Additionally, organic fluorophores and fluorescent proteins are bleached after 10(4)-10(5) excitation cycles. To overcome these restrictions, we synergistically combine the 3- to 7-fold improved axial resolution of 4Pi microscopy with the greatly enhanced photostability of semiconductor quantum dots. Co-localization studies of immunolabeled microtubules and mitochondria demonstrate the feasibility of this approach for routine biological measurements. In particular, we visualize the three-dimensional entanglement of the two networks with unprecedented detail.
Recommended Citation
Medda R,
Jakobs S,
Hell SW,
Bewersdorf J.
4Pi microscopy of quantum dot-labeled cellular structures. J Struct Biol 2006 Dec; 156(3):517-23.