Oocytes determine cumulus cell lineage in mouse ovarian follicles.
Document Type
Article
Publication Date
2007
Keywords
Benzamides, Cell-Lineage, Cells-Cultured, Dioxoles, Female, Fluorescent-Antibody-Technique, MAP-Kinase-Signaling-System, Mice, Oocytes, Ovarian-Follicle, Protein-Kinase-Inhibitors, RNA-Messenger, Smad2-Protein
First Page
1330
Last Page
1340
JAX Source
J Cell Sci 2007 Apr; 120(Pt.8):1330-40.
Abstract
The two principal functions of ovarian follicles are developmental and endocrine. The cumulus cells surrounding the oocyte are specialized to serve the development of the oocyte and steroidogenesis is a principal role of mural granulosa cells that line the follicle wall. The findings in this report demonstrate that oocytectomy or treatment with an inhibitor of SMAD2/3 activation results in decreased cumulus marker mRNA transcript levels and allows FSH to induce mural marker transcripts in cumulus cells. In addition, SMAD2/3 signaling is involved in enabling cumulus expansion and EGF-induced increases in Ptx3, Ptgs2 and Has2 mRNA levels. By contrast, follicle-stimulating hormone (FSH) stimulated expression of mural transcripts, but suppressed levels of cumulus transcripts. Thus, FSH and oocyte-stimulated SMAD2/3 signaling establish opposing gradients of influence in the follicle. These specify the mural and cumulus granulosa cell phenotypes that are pivotal for appropriate endocrine function and oocyte development.
Recommended Citation
Diaz FJ,
Wigglesworth K,
Eppig JJ.
Oocytes determine cumulus cell lineage in mouse ovarian follicles. J Cell Sci 2007 Apr; 120(Pt.8):1330-40.