Ligand-activated peroxisome proliferator activated receptor gamma alters placental morphology and placental fatty acid uptake in mice.
Document Type
Article
Publication Date
2007
Keywords
Fatty-Acids, Female, Fetal-Weight, Genotype, Hypoglycemic-Agents, Iodine-Radioisotopes, Iodobenzenes, Ligands, Male, Maternal-Fetal-Exchange, Mice-Inbred-C57BL, Mice-Mutant-Strains, PPAR-gamma, Placenta, Pregnancy, Thiazolidinediones
First Page
3625
Last Page
3634
JAX Source
Endocrinology 2007 Aug; 148(8):3625-34.
Abstract
The nuclear receptor peroxisome proliferator activated receptor gamma (PPARgamma) is essential for murine placental development. We previously showed that activation of PPARgamma in primary human trophoblasts enhances the uptake of fatty acids and alters the expression of several proteins associated with fatty acid trafficking. In this study we examined the effect of ligand-activated PPARgamma on placental development and transplacental fatty acid transport in wild-type (wt) and PPARgamma(+/-) embryos. We found that exposure of pregnant mice to the PPARgamma agonist rosiglitazone for 8 d (embryonic d 10.5-18.5) reduced the weights of wt, but not PPARgamma(+/-) placentas and embryos. Exposure to rosiglitazone reduced the thickness of the spongiotrophoblast layer and the surface area of labyrinthine vasculature, and altered expression of proteins implicated in placental development. The expression of fatty acid transport protein 1 (FATP1), FATP4, adipose differentiation related protein, S3-12, and myocardial lipid droplet protein was enhanced in placentas of rosiglitazone-treated wt embryos, whereas the expression of FATP-2, -3, and -6 was decreased. Additionally, rosiglitazone treatment was associated with enhanced accumulation of the fatty acid analog 15-(p-iodophenyl)-3-(R, S)-methyl pentadecanoic acid in the placenta, but not in the embryos. These results demonstrate that in vivo activation of PPARgamma modulates placental morphology and fatty acid accumulation.
Recommended Citation
Schaiff WT,
Knapp FF,
Barak Y,
Biron ST,
Nelson DM,
Sadovsky Y.
Ligand-activated peroxisome proliferator activated receptor gamma alters placental morphology and placental fatty acid uptake in mice. Endocrinology 2007 Aug; 148(8):3625-34.