Carbonic anhydrase related protein 8 mutation results in aberrant synaptic morphology and excitatory synaptic function in the cerebellum.
Document Type
Article
Publication Date
2007
Keywords
Cerebellar-Diseases, Cerebellum, Dendritic-Spines, Excitatory-Postsynaptic-Potentials, Mice-Inbred-C57BL, Mice-Mutant-Strains, Mice-Neurologic-Mutants, Microscopy-Electron, Nerve-Tissue-Proteins, Purkinje-Cells, Synapses, Tumor-Markers-Biological
First Page
161
Last Page
170
JAX Source
Mol Cell Neurosci 2007 May; 35(1):161-70.
Abstract
Carbonic anhydrase related protein 8 (Car8) is known to be abundantly expressed in Purkinje cells (PCs), and its genetic mutation causes a motor coordination defect. To determine the underlying mechanism, we analyzed the mouse cerebellum carrying a Car8 mutation. Electrophysiological analysis showed that spontaneous excitatory transmission was largely diminished while paired pulse ratio at parallel fiber-PC synapses was comparable to wild-type, suggesting functional synapses have normal release probability but the number of functional synapses may be lower in mutants. Light microscopic study revealed an abnormal extension of climbing fibers to the distal PC dendrites. At the ultrastructural level, we found numerous PC spines not forming synapses primarily in distal dendrites and occasionally multiple spines contacting a single varicosity. These abnormalities of parallel fiber-PC synapses may underlie the functional defect in excitatory transmission. Thus, Car8 plays a critical role in synaptogenesis and/or maintenance of proper synaptic morphology and function in the cerebellum.
Recommended Citation
Hirasawa M,
Xu X,
Trask RB,
Maddatu TP,
Johnson BA,
Naggert JK,
Nishina PM,
Ikeda A.
Carbonic anhydrase related protein 8 mutation results in aberrant synaptic morphology and excitatory synaptic function in the cerebellum. Mol Cell Neurosci 2007 May; 35(1):161-70.