Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters.
Document Type
Article
Publication Date
2008
Keywords
Apoptosis-Regulatory-Proteins, B-Lymphocytes, Cell-Survival, Embryonic-Stem-Cells, Fetus, Genes-Lethal, Heart-Septal-Defects-Ventricular, Lung-Diseases, Membrane-Proteins, Mice, MicroRNAs, Multigene-Family, Proto-Oncogene-Proteins, Sequence-Deletion
First Page
875
Last Page
886
JAX Source
Cell 2008 Mar; 132(5):875-86.
Abstract
miR-17 approximately 92, miR-106b approximately 25, and miR-106a approximately 363 belong to a family of highly conserved miRNA clusters. Amplification and overexpression of miR-1792 is observed in human cancers, and its oncogenic properties have been confirmed in a mouse model of B cell lymphoma. Here we show that mice deficient for miR-17 approximately 92 die shortly after birth with lung hypoplasia and a ventricular septal defect. The miR-17 approximately 92 cluster is also essential for B cell development. Absence of miR-17 approximately 92 leads to increased levels of the proapoptotic protein Bim and inhibits B cell development at the pro-B to pre-B transition. Furthermore, while ablation of miR-106b approximately 25 or miR-106a approximately 363 has no obvious phenotypic consequences, compound mutant embryos lacking both miR-106b approximately 25 and miR-17 approximately 92 die at midgestation. These results provide key insights into the physiologic functions of this family of microRNAs and suggest a link between the oncogenic properties of miR-17 approximately 92 and its functions during B lymphopoiesis and lung development.
Recommended Citation
Ventura A,
Young AG,
Winslow MM,
Lintault L,
Meissner A,
Erkeland SJ,
Newman J,
Bronson RT,
Crowley D,
Stone JR,
Jaenisch R,
Sharp PA,
Jacks T.
Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Cell 2008 Mar; 132(5):875-86.