The chromosome 11 region from strain 129 provides protection from sex reversal in XYPOS mice.
Document Type
Article
Publication Date
2008
Keywords
Chromosomes-Mammalian, Computational-Biology, DNA-Primers, Genotype, Male, Mice-Inbred-C57BL, Microsatellite-Repeats, Polymorphism-Single-Nucleotide, Sex-Determination-(Genetics), Sex-Reversal-Gonadal, Testis, Y-Chromosome
First Page
419
Last Page
427
JAX Source
Genetics 2008 May; 179(1):419-27.
Abstract
C57BL/6J (B6) mice containing the Mus domesticus poschiavinus Y chromosome, YPOS, develop ovarian tissue, whereas testicular tissue develops in DBA/2J or 129S1/SvImJ (129) mice containing the YPOS chromosome. To identify genes involved in sex determination, we used a congenic strain approach to determine which chromosomal regions from 129Sl/SvImJ provide protection against sex reversal in XYPOS mice of the C57BL/6J.129-YPOS strain. Genome scans using microsatellite and SNP markers identified a chromosome 11 region of 129 origin in C57BL/6J.129-YPOS mice. To determine if this region influenced testis development in XYPOS mice, two strains of C57BL/6J-YPOS mice were produced and used in genetic experiments. XYPOS adults homozygous for the 129 region had a lower incidence of sex reversal than XYPOS adults homozygous for the B6 region. In addition, many homozygous 129 XYPOS fetuses developed normal-appearing testes, an occurrence never observed in XYPOS mice of the C57BL/6J-YPOS strain. Finally, the amount of testicular tissue observed in ovotestes of heterozygous 129/B6 XYPOS fetuses was greater than the amount observed in ovotestes of homozygous B6 XYPOS fetuses. We conclude that a chromosome 11 locus derived from 129Sl/SvImJ essentially protects against sex reversal in XYPOS mice. A number of genes located in this chromosome 11 region are discussed as potential candidates.
Recommended Citation
Nikolova G,
Sinsheimer JS,
Eicher EM,
Vilain E.
The chromosome 11 region from strain 129 provides protection from sex reversal in XYPOS mice. Genetics 2008 May; 179(1):419-27.