Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library.
Chromosome-Mapping, Crosses-Genetic, Dinucleotide-Repeats, Female, Genomic-Library, Male, Microsatellite-Repeats, Polymorphism-(Genetics), Rabbits
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Anim Genet 2001 Oct; 32(5):308-12.
A genomic DNA library was produced from flow-sorted rabbit chromosome 1 and enriched for fragments containing CA-repeats. Clones containing CA-repeats were identified and primers for amplification of the microsatellite were developed after sequencing the clone. The degree of polymorphism was tested in rabbits from different breeds. This approach identified 12 microsatellite markers which could be used for studying linkage relationships in the progeny of an F(2)-intercross: (AX/JUxIIIVO/JU) F(2), and two backcrosses: (OS/JxX/J)X/J and (WH/JxX/J)X/J. Seven of these markers were mapped on chromosome 1.
Korstanje, R; Gillissen, G F.; den, Bieman M.; Versteeg, S A.; van, Oost B AU Fox; van, Lith H.; and van, Zutphen L., " Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library." (2001). Faculty Research 2000 - 2009. 327.