Cloning, expression, and chromosomal localization of a mouse gene homologous to the germ cell migration regulator wunen and to type 2 phosphatidic acid phosphatases.
Document Type
Article
Publication Date
2000
Keywords
Animal, Blotting-Northern, Cell-Movement, Chromosome-Mapping, Cloning-Molecular, Crosses-Genetic, Germ-Cells, Human, Insect-Proteins, Mice, Mice-Inbred-C57BL, Molecular-Sequence-Data, Phosphatidate-Phosphatase, Polymerase-Chain-Reaction, Sequence-Alignment, SUPPORT-U-S-GOVT-P-H-S
First Page
142
Last Page
144
JAX Source
Genomics 2000 Jan; 63(1):142-4.
Grant
RO1NS36437/NS/NINDS, RO1HD34883/HD/NICHD, CA34196/CA/NCI
Abstract
The wunen gene of Drosophila melanogaster encodes a multipass membrane-spanning protein that negatively regulates primordial germ cell migration. Here we describe the cloning of a mouse gene that encodes a protein homologous to wunen and to the Type 2 phosphatidic acid phosphatases. This gene encodes a 251-amino-acid protein that most closely resembles the human Type 2 phosphatidic acid phosphatase PAP-2c. Northern blot analysis revealed the presence of a single 1.9-kb Ppap2c transcript. The Ppap2c gene was localized to the central portion of mouse Chromosome 10 by interspecific backcross analysis. Copyright 2000 Academic Press.
Recommended Citation
Zhang N,
Copeland NG,
Gilbert DJ,
Jenkins NA,
Gridley T.
Cloning, expression, and chromosomal localization of a mouse gene homologous to the germ cell migration regulator wunen and to type 2 phosphatidic acid phosphatases. Genomics 2000 Jan; 63(1):142-4.