Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs.
Document Type
Article
Publication Date
2002
Keywords
Amino-Acid-Motifs, Chromosomes-Mammalian, Cloning-Molecular, DNA-Complementary, Databases-Genetic, Expressed-Sequence-Tags, Genes, Genomics, Membrane-Proteins, Mice, Physical-Chromosome-Mapping, Protein-Structure-Tertiary, Proteome, RNA-Antisense, RNA-Messenger, RNA-Untranslated
First Page
563
Last Page
573
JAX Source
Nature 2002 Dec; 420(6915) 563-73.
Abstract
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.
Recommended Citation
Okazaki Y,
Baldarelli R,
Hill DP,
Bult C,
Hume DA,
Quackenbush J,
Blake JA,
Bradt D,
Corbani LE,
Frazer KS,
Jackson IJ,
Maltais L,
Reed DJ,
Ringwald M,
Lander ES.
Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature 2002 Dec; 420(6915) 563-73.