Metaphase FISHing of transgenic mice recommended: FISH and SKY define BAC-mediated balanced translocation.
Document Type
Article
Publication Date
2003
Keywords
Chromosomes-Artificial-Bacterial, Comparative-Study, DNA-Primers, Gene-Expression, Gene-Transfer-Techniques, In-Situ-Hybridization-Fluorescence, Karyotyping, Metaphase, Mice-Transgenic, Polymerase-Chain-Reaction, Reverse-Transcriptase-Polymerase-Chain-Reaction
First Page
134
Last Page
141
JAX Source
Genesis 2003 Jul; 36(3):134-41.
Abstract
The evolving trend to use larger transgenes and their associated increased chance of unexpected genetic events mandates more careful characterization of transgenic mice. In characterizing our five new mouse strains transgenic for the BAC, bEMS4, we have identified the highest copy number reported to date: the stable incorporation of approximately 40 copies of a 194-kb expressed transgene in a single insertion site. We caution, however, that standard molecular techniques failed to identify a balanced translocation in another strain, and an inappropriate site of insertion in a third. Molecular cytogenetic analysis using metaphase FISH was the minimum level of characterization needed to reveal these unexpected genetic events. In addition, we combined FISH and SKY to identify the transgene at the breakpoints of the balanced translocation, t(3;9). This is the first description of a BAC-mediated chromosomal rearrangement and the first application of SKY to identify transgene-induced chromosomal rearrangements.
Recommended Citation
Abrahams BS,
Chong AC,
Nisha M,
Milette D,
Brewster DA,
Berry ML,
Muratkhodjaev F,
Mai S,
Rajcan SE,
Simpson EM.
Metaphase FISHing of transgenic mice recommended: FISH and SKY define BAC-mediated balanced translocation. Genesis 2003 Jul; 36(3):134-41.