Transgenic Cre expression mice for generation of erythroid-specific gene alterations.
Document Type
Article
Publication Date
2004
First Page
1
Last Page
9
JAX Source
Genesis 2004 May; 39(1):1-9.
Abstract
Transgenic mice that express Cre recombinase in erythroid cell lineages were developed so that genes affecting erythropoiesis/hematopoiesis may be altered without necessarily affecting fetus viability. A micro-LCR cassette-beta-globin promoter-Cre recombinase gene (microLCR-betapr-Cre) construct was synthesized and used to generate transgenic mice. Concurrently, we produced mice containing a microLCR-loxP-flanked beta sickle gene (microLCR-loxP-beta(S)-loxP) construct. microLCR-betapr-Cre mice with intact transgenes in variable copy number were identified. Cre expression was assessed by RNAse protection and RT-PCR. Cre function was ascertained by breeding to microLCR-loxP-beta(S)-loxP mice. We demonstrate that beta(S) expression was not detected in the blood of bigenics, but the gene was present in nonerythroid cells. Thus, excision of the loxP-flanked beta(S) gene was restricted to erythroid cell lineages.
Recommended Citation
Peterson KR,
Fedosyuk H,
Zelenchuk L,
Nakamoto B,
Yannaki E,
Stamatoyannopoulos G,
Ciciotte S,
Peters LL,
Scott LM,
Papayannopoulou T.
Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 2004 May; 39(1):1-9.