Mutation discovery in mice by whole exome sequencing.
Document Type
Article
Publication Date
9-14-2011
JAX Source
Genome Biol 2011 Sep 14; 12(9):R86.
PMID
21917142
Volume
12
Issue
9
First Page
86
Last Page
86
ISSN
1465-6914
Abstract
We report the development and optimization of reagents for in-solution, hybridization-based capture of the mouse exome. By validating this approach in a multiple inbred strains and in novel mutant strains, we show that whole exome sequencing is a robust approach for discovery of putative mutations, irrespective of strain background. We found strong candidate mutations for the majority of mutant exomes sequenced, including new models of orofacial clefting, urogenital dysmorphology, kyphosis and autoimmune hepatitis.
Recommended Citation
Fairfield H,
Gilbert G,
Barter M,
Corrigan R,
Curtain M,
Ding Y,
D'Ascenzo M,
Gerhardt D,
He C,
Huang W,
Richmond T,
Rowe L,
Probst F,
Bergstrom D,
Murray S,
Bult C,
Richardson J,
Kile B,
Gut I,
Hager J,
Sigurdsson S,
Mauceli E,
Di Palma F,
Lindblad-Toh K,
Cunningham M,
Cox T,
Justice M,
Spector M,
Lowe S,
Albert T,
Donahue L,
Jeddeloh J,
Shendure J,
Reinholdt L.
Mutation discovery in mice by whole exome sequencing. Genome Biol 2011 Sep 14; 12(9):R86.