Structured illumination microscopy of autofluorescent aggregations in human tissue.
Document Type
Article
Publication Date
6-2011
Keywords
Structured illumination; Microscopy; Autofluorescence; Retinal pigment epithelium; Interferometer
JAX Source
Micron 2011 Jun; 42(4):330-335.
PMID
20926302
ISSN
1878-4291
Abstract
Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SIM) using a specially designed microscope setup. In this microscope the structured illumination was generated with a Twyman-Green Interferometer. This SIM technique allowed us to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. In this work the unique setup of the microscope made possible the application of SIM with three different excitation wavelengths (488, 568 and 647nm), thus enabling us to gather spectral information about the autofluorescence signal.
Recommended Citation
Best G,
Amberger R,
Baddeley D,
Ach T,
Dithmar S,
Heintzmann R,
Cremer C.
Structured illumination microscopy of autofluorescent aggregations in human tissue. Micron 2011 Jun; 42(4):330-335.