Imaging label-free intracellular structures by localisation microscopy.
Document Type
Article
Publication Date
6-2011
Keywords
Sub-diffraction limit microscopy; Localisation microscopy; Spectral Precision Distance Microscopy (SPDM); Fluorescence
JAX Source
Micron 2011 Jun; 42(4):348-52.
PMID
20538472
ISSN
1878-4291
Abstract
Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200nm laterally and 600nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the lambda/10 range ( approximately 50nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions.
Recommended Citation
Kaufmann R,
Müller P,
Hausmann M,
Cremer C.
Imaging label-free intracellular structures by localisation microscopy. Micron 2011 Jun; 42(4):348-52.