Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn.
Document Type
Article
Publication Date
12-2011
Keywords
Animals, Base Sequence, Disease Models, Animal, Down Syndrome, Female, Gene Dosage, Genotype, High-Throughput Nucleotide Sequencing, Male, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Polymorphism, Single Nucleotide, Sequence Alignment, Sequence Analysis, DNA, Translocation, Genetic, Trisomy
JAX Source
Mamm Genome 2011 Dec; 22(11-12):685-91.
PMID
21953412
Volume
22
Issue
11-12
First Page
685
Last Page
691
ISSN
1432-1777
Abstract
Ts65Dn is a mouse model of Down syndrome: a syndrome that results from chromosome (Chr) 21 trisomy and is associated with congenital defects, cognitive impairment, and ultimately Alzheimer's disease. Ts65Dn mice have segmental trisomy for distal mouse Chr 16, a region sharing conserved synteny with human Chr 21. As a result, this strain harbors three copies of over half of the human Chr 21 orthologs. The trisomic segment of Chr 16 is present as a translocation chromosome (Mmu17(16)), with breakpoints that have not been defined previously. To molecularly characterize the Chrs 16 and 17 breakpoints on the translocation chromosome in Ts65Dn mice, we used a selective enrichment and high-throughput paired-end sequencing approach. Analysis of paired-end reads flanking the Chr 16, Chr 17 junction on Mmu17(16) and de novo assembly of the reads directly spanning the junction provided the precise locations of the Chrs 16 and 17 breakpoints at 84,351,351 and 9,426,822 bp, respectively. These data provide the basis for low-cost, highly efficient genotyping of Ts65Dn mice. More importantly, these data provide, for the first time, complete characterization of gene dosage in Ts65Dn mice.
Recommended Citation
Reinholdt L,
Ding Y,
Gilbert G,
Gilbert G,
Czechanski A,
Solzak J,
Roper R,
Johnson M,
Donahue L,
Lutz C,
Davisson M.
Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn. Mamm Genome 2011 Dec; 22(11-12):685-91.