Inhibition of NF-kappaB signaling retards eosinophilic dermatitis in SHARPIN-deficient mice.

Document Type

Article

Publication Date

2011

Keywords

Boronic-Acids, Dermatitis, Eosinophilia, Eosinophils, Gene-Expression-Profiling, Immunohistochemistry, Mice-Inbred-C57BL, Mice-Mutant-Strains, NF-kappa-B-p50-Subunit, Nerve-Tissue-Proteins, Phenotype, Phosphorylation, Protease-Inhibitors, Pyrazines, Signal-Transduction, Transcription-Factor-RelA

JAX Source

J Invest Dermatol 2011 Jan; 131(1):141-9.

First Page

141

Last Page

149

Abstract

The NF-kappaB pathway performs pivotal roles in diverse physiological processes such as immunity, inflammation, proliferation, and apoptosis. NF-kappaB is kept inactive in the cytoplasm through association with inhibitors (IkappaB), and translocates to the nucleus to activate its target genes after the IkappaBs are phosphorylated and degraded. Here, we demonstrate that loss of function of SHANK-associated RH domain interacting protein (SHARPIN) leads to activation of NF-kappaB signaling in skin, resulting in the development of an idiopathic hypereosinophilic syndrome (IHES) with eosinophilic dermatitis in C57BL/KaLawRij-Sharpin(cpdm)/RijSunJ mice, and clonal expansion of B-1 B cells and CD3(+)CD4(-)CD8(-) T cells. Transcription profiling in skin revealed constitutive activation of classical NF-kappaB pathways, predominantly by overexpressed members of IL1 family. Compound-null mutants for both the IL1 receptor accessory protein (Il1rap(tm1Roml)) and SHARPIN (Sharpin(cpdm)) resulted in mice having decreased skin disease severity. Inhibition of IkappaBA degradation by the proteasome inhibitor bortezomib alleviated the dermatitis in Sharpin(cpdm) mice. These results indicate that absence of SHARPIN causes IHES with eosinophilic dermatitis by NF-kappaB activation, and bortezomib may be an effective treatment for skin problems of IHES.

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