Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource.
Document Type
Article
Publication Date
11-20-2012
JAX Source
Nat Commun 2012 Nov 20; 3:1218.
PMID
23169059
Volume
3
First Page
1218
Last Page
1218
ISSN
2041-1723
Abstract
Full realization of the value of the loxP-flanked alleles generated by the International Knockout Mouse Consortium will require a large set of well-characterized cre-driver lines. However, many cre driver lines display excision activity beyond the intended tissue or cell type, and these data are frequently unavailable to the potential user. Here we describe a high-throughput pipeline to extend characterization of cre driver lines to document excision activity in a wide range of tissues at multiple time points and disseminate these data to the scientific community. Our results show that the majority of cre strains exhibit some degree of unreported recombinase activity. In addition, we observe frequent mosaicism, inconsistent activity and parent-of-origin effects. Together, these results highlight the importance of deep characterization of cre strains, and provide the scientific community with a critical resource for cre strain information.
Recommended Citation
Heffner C,
Herbert Pratt C,
Babiuk R,
Sharma Y,
Rockwood S,
Donahue L,
Eppig J,
Murray S.
Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource. Nat Commun 2012 Nov 20; 3:1218.