Gene expression in mouse oocytes by RNA-Seq.
Document Type
Article
Publication Date
2012
Keywords
Animals, DNA, Complementary, Female, Gene Expression Profiling, Gene Library, High-Throughput Nucleotide Sequencing, Mice, Nucleic Acid Amplification Techniques, Oocyte Retrieval, Oocytes, Quality Control, RNA, Sequence Analysis, RNA
JAX Location
Reprint Collection
JAX Source
Methods Mol Biol 2012; 825:237-51.
PMID
22144249
Volume
825
First Page
237
Last Page
251
ISSN
1940-6029
Abstract
Second-generation sequencers such as the Illumina GAIIx make possible the study of all transcribed loci in a genome across an almost endless dynamic range. Although typical protocols call for starting from at least 1 μg of total RNA, this is not possible when studying small tissues or rare cell types. This chapter explains how to prepare Illumina sequencing libraries from mouse oocytes. The protocol is also suitable for mural and cumulus cells, flow sorted or laser captured cells.
Recommended Citation
Antoniou E,
Taft R.
Gene expression in mouse oocytes by RNA-Seq. Methods Mol Biol 2012; 825:237-51.