The zebrafish reference genome sequence and its relationship to the human genome.
Document Type
Article
Publication Date
4-25-2013
Keywords
Animals, Chromosomes, Conserved Sequence, Evolution, Molecular, Female, Genes, Genome, Genome, Human, Genomics, Humans, Male, Meiosis, Molecular Sequence Annotation, Pseudogenes, Reference Standards, Sex Determination Processes, Zebrafish, Zebrafish Proteins
JAX Source
Nature 2013 Apr 25; 496(7446):498-503.
Volume
496
Issue
7446
First Page
498
Last Page
503
ISSN
1476-4687
PMID
23594743
Abstract
Zebrafish have become a popular organism for the study of vertebrate gene function. The virtually transparent embryos of this species, and the ability to accelerate genetic studies by gene knockdown or overexpression, have led to the widespread use of zebrafish in the detailed investigation of vertebrate gene function and increasingly, the study of human genetic disease. However, for effective modelling of human genetic disease it is important to understand the extent to which zebrafish genes and gene structures are related to orthologous human genes. To examine this, we generated a high-quality sequence assembly of the zebrafish genome, made up of an overlapping set of completely sequenced large-insert clones that were ordered and oriented using a high-resolution high-density meiotic map. Detailed automatic and manual annotation provides evidence of more than 26,000 protein-coding genes, the largest gene set of any vertebrate so far sequenced. Comparison to the human reference genome shows that approximately 70% of human genes have at least one obvious zebrafish orthologue. In addition, the high quality of this genome assembly provides a clearer understanding of key genomic features such as a unique repeat content, a scarcity of pseudogenes, an enrichment of zebrafish-specific genes on chromosome 4 and chromosomal regions that influence sex determination. Nature 2013 Apr 25; 496(7446):498-503.
Recommended Citation
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The zebrafish reference genome sequence and its relationship to the human genome. Nature 2013 Apr 25; 496(7446):498-503.