TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes.
Document Type
Article
Publication Date
1-15-2013
Keywords
Animals, B-Lymphocytes, Bone Marrow Cells, Cell Differentiation, Cell Lineage, Erythroid Cells, Gene Deletion, Gene Expression Regulation, Developmental, Granulocyte-Macrophage Progenitor Cells, Granulocytes, Hematopoiesis, Mice, Mice, Inbred C57BL, Mice, Knockout, Myelopoiesis, Spleen, Transcription Elongation, Genetic, Transcription Factors
JAX Source
Dev Biol 2013 Jan 15; 373(2):422-30.
Volume
373
Issue
2
First Page
422
Last Page
430
ISSN
1095-564X
PMID
23159334
Abstract
Transcriptional regulators play critical roles in the regulation of cell fate during hematopoiesis. Previous studies in zebrafish have identified an essential role for the transcriptional intermediary factor TIF1γ in erythropoiesis by regulating the transcription elongation of erythroid genes. To study if TIF1γ plays a similar role in murine erythropoiesis and to assess its function in other blood lineages, we generated mouse models with hematopoietic deletion of TIF1γ. Our results showed a block in erythroid maturation in the bone marrow following tif1γ deletion that was compensated with enhanced spleen erythropoiesis. Further analyses revealed a defect in transcription elongation of erythroid genes in the bone marrow. In addition, loss of TIF1γ resulted in defects in other blood compartments, including a profound loss of B cells, a dramatic expansion of granulocytes and decreased HSC function. TIF1γ exerts its functions in a cell-autonomous manner as revealed by competitive transplantation experiments. Our study therefore demonstrates that TIF1γ plays essential roles in multiple murine blood lineages and that its function in transcription elongation is evolutionally conserved. Dev Biol 2013 Jan 15; 373(2):422-30.
Recommended Citation
Bai X,
Trowbridge JJ,
Riley E,
Lee J,
DiBiase A,
Kaartinen V,
Orkin S,
Zon L.
TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes. Dev Biol 2013 Jan 15; 373(2):422-30.