Dendritic cell-based therapeutic vaccine elicits polyfunctional HIV-specific T-cell immunity associated with control of viral load.
Document Type
Article
Publication Date
9-2014
JAX Source
Eur J Immunol 2014 Sep; 44(9):2802-10.
Volume
44
Issue
9
First Page
2802
Last Page
2810
ISSN
1521-4141
PMID
25042008
Abstract
Efforts aimed at restoring robust immune responses limiting human immunodeficiency virus (HIV)-1 replication therapeutically are warranted. We report that vaccination with dendritic cells generated ex vivo and loaded with HIV lipopeptides in patients (n = 19) on antiretroviral therapy was well tolerated and immunogenic. Vaccination increased: (i) the breadth of the immune response from 1 (1-3) to 4 (2-5) peptide-pool responses/patient (p = 0.009); (ii) the frequency of functional T cells (producing at least two cytokines among IFN-γ, TNF-α, and IL-2) from 0.026 to 0.32% (p = 0.002) and from 0.26 to 0.35% (p = 0.005) for CD4(+) and CD8(+) T cells, respectively; and (iii) the breadth of cytokines secreted by PBMCs upon antigen exposure, including IL-2, IFN-γ, IL-21, IL-17, and IL-13. Fifty percent of patients experienced a maximum of viral load (VL) 1 log10 lower than the other half following antiretroviral treatment interruption. An inverse correlation was found between the maximum of VL and the frequency of polyfunctional CD4(+) T cells (p = 0.007), production of IL-2 (p = 0.006), IFN-γ (p = 0.01), IL-21 (p = 0.006), and IL-13 (p = 0.001). These results suggest an association between vaccine responses and a better control of viral replication. These findings will help in the development of strategies for a functional cure for HIV infection. Eur J Immunol 2014 Sep; 44(9):2802-10.
Recommended Citation
Lévy Y,
Thiébaut R,
Montes M,
Lacabaratz C,
Sloan L,
King B,
Pérusat S,
Harrod C,
Cobb A,
Roberts L,
Surenaud M,
Boucherie C,
Zurawski S,
Delaugerre C,
Richert L,
Chêne G,
Banchereau J,
Palucka K.
Dendritic cell-based therapeutic vaccine elicits polyfunctional HIV-specific T-cell immunity associated with control of viral load. Eur J Immunol 2014 Sep; 44(9):2802-10.