Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines.
Document Type
Article
Publication Date
10-22-2014
JAX Source
Nat Commun 2014 Oct 22; 5:5283.
Volume
5
First Page
5283
Last Page
5283
ISSN
2041-1723
PMID
25335753
Abstract
The mechanisms by which microbial vaccines interact with human APCs remain elusive. Herein, we describe the transcriptional programs induced in human DCs by pathogens, innate receptor ligands and vaccines. Exposure of DCs to influenza, Salmonella enterica and Staphylococcus aureus allows us to build a modular framework containing 204 transcript clusters. We use this framework to characterize the responses of human monocytes, monocyte-derived DCs and blood DC subsets to 13 vaccines. Different vaccines induce distinct transcriptional programs based on pathogen type, adjuvant formulation and APC targeted. Fluzone, Pneumovax and Gardasil, respectively, activate monocyte-derived DCs, monocytes and CD1c+ blood DCs, highlighting APC specialization in response to vaccines. Finally, the blood signatures from individuals vaccinated with Fluzone or infected with influenza reveal a signature of adaptive immunity activation following vaccination and symptomatic infections, but not asymptomatic infections. These data, offered with a web interface, may guide the development of improved vaccines. Nat Commun 2014 Oct 22; 5:5283.
Recommended Citation
Banchereau R,
Baldwin N,
Cepika A,
Athale S,
Xue Y,
Yu C,
Metang P,
Cheruku A,
Berthier I,
Gayet I,
Wang Y,
Ohouo M,
Snipes L,
Xu H,
Obermoser G,
Blankenship D,
Oh S,
Ramilo O,
Chaussabel D,
Banchereau J,
Palucka K,
Pascual V.
Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines. Nat Commun 2014 Oct 22; 5:5283.