Germline Quality Control: eEF2K Stands Guard to Eliminate Defective Oocytes.
Document Type
Article
Publication Date
3-10-2014
JAX Source
Dev Cell 2014 Mar 10; 28(5):561-72.
ISSN
1878-1551
PMID
24582807
Abstract
The control of germline quality is critical to reproductive success and survival of a species; however, the mechanisms underlying this process remain unknown. Here, we demonstrate that elongation factor 2 kinase (eEF2K), an evolutionarily conserved regulator of protein synthesis, functions to maintain germline quality and eliminate defective oocytes. We show that disruption of eEF2K in mice reduces ovarian apoptosis and results in the accumulation of aberrant follicles and defective oocytes at advanced reproductive age. Furthermore, the loss of eEF2K in Caenorhabditis elegans results in a reduction of germ cell death and significant decline in oocyte quality and embryonic viability. Examination of the mechanisms by which eEF2K regulates apoptosis shows that eEF2K senses oxidative stress and quickly downregulates short-lived antiapoptotic proteins, XIAP and c-FLIPL by inhibiting global protein synthesis. These results suggest that eEF2K-mediated inhibition of protein synthesis renders cells susceptible to apoptosis and functions to eliminate suboptimal germ cells. Dev Cell 2014 Mar 10; 28(5):561-72.
Recommended Citation
Chu H,
Liao Y,
Novak J,
Hu Z,
Merkin J,
Shymkiv Y,
Braeckman B,
Dorovkov M,
Nguyen A,
Clifford P,
Nagele R,
Harrison DE,
Ellis R,
Ryazanov A.
Germline Quality Control: eEF2K Stands Guard to Eliminate Defective Oocytes. Dev Cell 2014 Mar 10; 28(5):561-72.