A promoter-level mammalian expression atlas.
Document Type
Article
Publication Date
3-27-2014
JAX Source
Nature 2014 Mar 27; 507(7493):462-70.
Volume
507
Issue
7493
First Page
462
Last Page
470
ISSN
1476-4687
PMID
24670764
Abstract
Regulated transcription controls the diversity, developmental pathways and spatial organization of the hundreds of cell types that make up a mammal. Using single-molecule cDNA sequencing, we mapped transcription start sites (TSSs) and their usage in human and mouse primary cells, cell lines and tissues to produce a comprehensive overview of mammalian gene expression across the human body. We find that few genes are truly 'housekeeping', whereas many mammalian promoters are composite entities composed of several closely separated TSSs, with independent cell-type-specific expression profiles. TSSs specific to different cell types evolve at different rates, whereas promoters of broadly expressed genes are the most conserved. Promoter-based expression analysis reveals key transcription factors defining cell states and links them to binding-site motifs. The functions of identified novel transcripts can be predicted by coexpression and sample ontology enrichment analyses. The functional annotation of the mammalian genome 5 (FANTOM5) project provides comprehensive expression profiles and functional annotation of mammalian cell-type-specific transcriptomes with wide applications in biomedical research. Nature 2014 Mar 27; 507(7493):462-70.
Recommended Citation
Consortium F,
Blake JA.
A promoter-level mammalian expression atlas. Nature 2014 Mar 27; 507(7493):462-70.