Title
CRISPR-Cas9-mediated genome editing and guide RNA design.
Document Type
Article
Publication Date
10-2015
JAX Source
Mamm Genome 2015 Oct; 26(9-10):501-10.
Volume
26
Issue
9-10
First Page
501
Last Page
510
ISSN
1432-1777
PMID
25991564
Abstract
CRISPR and CRISPR-associated (Cas) proteins, which in nature comprise the RNA-based adaptive immune system in bacteria and archaea, have emerged as particularly powerful genome editing tools owing to their unrivaled ease of use and ability to modify genomes across mammalian model systems. As such, the CRISPR-Cas9 system holds promise as a "system of choice" for functional mammalian genetic studies across biological disciplines. Here we briefly review this fast moving field, introduce the CRISPR-Cas9 system and its application to genome editing, with a focus on the basic considerations in designing the targeting guide RNA sequence. Mamm Genome 2015 Oct; 26(9-10):501-10.
Recommended Citation
Wiles MV,
Qin W,
Cheng AW,
Wang H.
CRISPR-Cas9-mediated genome editing and guide RNA design. Mamm Genome 2015 Oct; 26(9-10):501-10.