Document Type
Article
Publication Date
8-30-2016
JAX Source
Cell Rep 2016 Aug 30; 16(9):2472-85
Volume
16
Issue
9
First Page
2472
Last Page
2485
ISSN
2211-1247
PMID
27545885
Grant
1R21AI110776-01
Abstract
Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ DCs migrated into the CD4+ T cell area, whereas XCR1+ DCs migrated into the CD8+ T cell area. Thus, the post-immunization location of each DC subset correlated with the T cell lineage it preferentially primes. Preventing this co-localization selectively impaired either CD4+ or CD8+ T cell immunity to blood-borne antigens. Cell Rep 2016 Aug 30; 16(9):2472-85.
Recommended Citation
Calabro S,
Liu D,
Gallman A,
Nascimento M,
Yu Z,
Zhang T,
Chen P,
Zhang B,
Xu L,
Gowthaman U,
Krishnaswamy J,
Haberman A,
Williams A,
Eisenbarth S.
Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity. Cell Rep 2016 Aug 30; 16(9):2472-85