Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity.
Cell Rep 2016 Aug 30; 16(9):2472-85
Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ DCs migrated into the CD4+ T cell area, whereas XCR1+ DCs migrated into the CD8+ T cell area. Thus, the post-immunization location of each DC subset correlated with the T cell lineage it preferentially primes. Preventing this co-localization selectively impaired either CD4+ or CD8+ T cell immunity to blood-borne antigens. Cell Rep 2016 Aug 30; 16(9):2472-85.
Calabro, Samuele; Liu, Dong; Gallman, Antonia; Nascimento, Manuela Sales L; Yu, Zizi; Zhang, Ting-Ting; Chen, Pei; Zhang, Biyan; Xu, Lan; Gowthaman, Uthaman; Krishnaswamy, Jayendra Kumar; Haberman, Ann M; Williams, Adam; and Eisenbarth, Stephanie C, "Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity." (2016). Faculty Research 2016. 198.